Induced endothelial cell migration
The function tested VEGF-A is cloned into immortalised human fibroblasts, which form the core of the aggregate tissue. The high VEGF-A levels induce migration of HMVEC-L. As a result, they become aligned at the source of the VEGF-A (Figure 1).
Small molecules, peptides and recombinant viruses penetrate the tissue (Figure 2)
Various types of angiogenesis inhibitors have been developed that are currently used in therapy. Our tissue system is suitable to test their efficacy of existing agents and drugs, aswell as those under development.
Inhibition of tube formation is visualised
Inhibition of endothelial cell migration, proliferation and allignment can be tested using various techniques, including: qRT-PCR, immunohistochemistry etc.